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KMID : 1094720070120060601
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 6 p.601 ~ p.609
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Effects of mechanical stimulation on the proliferation of bone marrow-derived human mesenchymal stem cells
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Choi Kyung-Min
Seo Young-Kwon
Yoon Hee-Hoon
Song Kye-Yong
Kwon Soon-Yong
Lee Hwa-Sung
Park Jung-Keug
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Abstract
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To support and enhance thein vitro growth and activity of mesenchymal stem cells (MSCs), the cell culture medium may be supplemented with various proteins and factors to mimic the physiological environment in which the cells optimally proliferate and differentiate. In this study, the effects of mechanical factors on cellular metabolic responses were investigated experimentally using a bioreactor. The effects of various chemical factors, such as growth factors, cytokines, and hormones, were also investigated. Based on previous reports demonstrating the important roles of mechanical factors in the growth and activity of MSCs, we sought to evaluate the effects of mechanical stimuli on the proliferation of bone marrow-derived MSCs using a cell training bioreactor that imposed cyclic mechanical stretch, with parameters of 240 min/day, 0.03 Hz, and 5?15% strain. The application of cyclic stretch (5?15% strain) to the MSCs enhanced their proliferation during the early stage (3 days), but not the late stage (14 days), of batch culture. Mechanical stretch did not increase the release of lactate dehydrogenase (LDH) from the MSCs during culture. Appropriate levels of mechanical stretch (5?10% strain) increased collagen synthesis, but did not alter MSC surface antigen expression. It is thought that the appropriate level of mechanical stretch was able to serve as a potent positive modulator of MSC proliferation during the initial stages of culture.
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KEYWORD
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mechanical stimulation, proliferation, mesenchymal stem cel, bone marrow
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